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5 ethynyl 2 deoxyuridine edu  (Jena Bioscience)


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    Jena Bioscience 5 ethynyl 2 deoxyuridine edu
    5 Ethynyl 2 Deoxyuridine Edu, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5+edu/5-Ethynyl-2'-deoxyuridine/pm42731029-284-186-188
    Average 95 stars, based on 66 article reviews
    5 ethynyl 2 deoxyuridine edu - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    EdU Assay:

    Article Title: Live imaging of human airway epithelial repair in precision-cut lung slices after targeted cell damage
    Article Snippet: .. For the EdU assay, hPCLS media was supplemented with 10 μM 5-EdU (Jena Bioscience, Germany) and hPCLS were incubated at 37 °C, 5 % CO 2 for 2 h prior to subsequent laser-based cell ablation and live cell imaging. .. For the EdU assay, hPCLS media was supplemented with 10 μM 5-EdU (Jena Bioscience, Germany) and hPCLS were incubated at 37 °C, 5 % CO 2 for 2 h prior to subsequent laser-based cell ablation and live cell imaging.

    Incubation:

    Article Title: Live imaging of human airway epithelial repair in precision-cut lung slices after targeted cell damage
    Article Snippet: .. For the EdU assay, hPCLS media was supplemented with 10 μM 5-EdU (Jena Bioscience, Germany) and hPCLS were incubated at 37 °C, 5 % CO 2 for 2 h prior to subsequent laser-based cell ablation and live cell imaging. .. For the EdU assay, hPCLS media was supplemented with 10 μM 5-EdU (Jena Bioscience, Germany) and hPCLS were incubated at 37 °C, 5 % CO 2 for 2 h prior to subsequent laser-based cell ablation and live cell imaging.

    Live Cell Imaging:

    Article Title: Live imaging of human airway epithelial repair in precision-cut lung slices after targeted cell damage
    Article Snippet: .. For the EdU assay, hPCLS media was supplemented with 10 μM 5-EdU (Jena Bioscience, Germany) and hPCLS were incubated at 37 °C, 5 % CO 2 for 2 h prior to subsequent laser-based cell ablation and live cell imaging. .. For the EdU assay, hPCLS media was supplemented with 10 μM 5-EdU (Jena Bioscience, Germany) and hPCLS were incubated at 37 °C, 5 % CO 2 for 2 h prior to subsequent laser-based cell ablation and live cell imaging.



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    Cellular expression and functional validation of CTHRC1 in vitro . (A–C) Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis of SERPINE1, CTHRC1, and EGR1 in GES-1, AGS, HGC-27, and MKN-45 cells. (D) qRT-PCR screening of three CTHRC1-targeting short hairpin RNAs (shRNAs) in HGC-27 and AGS cells. (E) Western blot validation of CTHRC1 knockdown; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was the loading control. (F, G) Cell Counting Kit-8 (CCK-8) proliferation assays. (H, I) Representative 5-ethynyl-2′-deoxyuridine (EdU) staining and quantification; scale bar, 100 µm. (J, K) Colony-formation assays and quantification; scale bar, 10 mm. (L–N) Wound-healing assays and migration-rate quantification; scale bar, 100 µm. (O–R) Transwell migration/invasion assays and quantification; scale bar, 100 µm. Data are mean ± standard deviation (SD) from three independent biological experiments. (A–D) were analyzed by one-way analysis of variance (ANOVA) with Dunnett correction, panels F and G by two-way ANOVA with multiple-comparison correction, and two-group endpoint comparisons by two-sided Student’s t-tests. ***P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: A palmitoylation-associated three-gene signature predicts prognosis, immune features, and drug sensitivity in gastric cancer: functional validation of CTHRC1

    doi: 10.3389/fimmu.2026.1893438

    Figure Lengend Snippet: Cellular expression and functional validation of CTHRC1 in vitro . (A–C) Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis of SERPINE1, CTHRC1, and EGR1 in GES-1, AGS, HGC-27, and MKN-45 cells. (D) qRT-PCR screening of three CTHRC1-targeting short hairpin RNAs (shRNAs) in HGC-27 and AGS cells. (E) Western blot validation of CTHRC1 knockdown; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was the loading control. (F, G) Cell Counting Kit-8 (CCK-8) proliferation assays. (H, I) Representative 5-ethynyl-2′-deoxyuridine (EdU) staining and quantification; scale bar, 100 µm. (J, K) Colony-formation assays and quantification; scale bar, 10 mm. (L–N) Wound-healing assays and migration-rate quantification; scale bar, 100 µm. (O–R) Transwell migration/invasion assays and quantification; scale bar, 100 µm. Data are mean ± standard deviation (SD) from three independent biological experiments. (A–D) were analyzed by one-way analysis of variance (ANOVA) with Dunnett correction, panels F and G by two-way ANOVA with multiple-comparison correction, and two-group endpoint comparisons by two-sided Student’s t-tests. ***P < 0.001.

    Article Snippet: DNA synthesis was assessed using a BeyoClick 5-ethynyl-2′-deoxyuridine (EdU)-594 Cell Proliferation Kit (Beyotime, C0078S).

    Techniques: Expressing, Functional Assay, Biomarker Discovery, In Vitro, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Analysis, Screening, Western Blot, Knockdown, Control, Cell Counting, CCK-8 Assay, Staining, Colony Assay, Migration, Standard Deviation, Comparison